To be recognized
by the eukaryotic machinery, a gene should be theoreticaly monocystronic,
have a eukaryotic promoter (CMV, SV40, etc.), an intron at its
5' end, and a poly(A) tail.
yields are very low. The few transfected cells have to be detected.
Here transfected eukaryotic cell would express ß-galactosidase
and show a ß-galactosidase activity. This activity will
be recognized by a color reaction which will stain the cells
in blue with specific reagent.
We will detect
the cells which synthesize ß-galactosidase with a microscope,
using a staining reaction.
Etienne DE PLAEN)