(the gel could be loaded in capillaires)
These 21 sequences
are separated in a denaturing polyacrylamide electrophoresis.
(denaturing, to separate the template).
will move in the gel as a function of its size, the shortest
They will pass
according to their sizes in front of a excitatory laser beam
and a fluorescence detector. As a sequence passes through the
excitatory laser beam, it fluoresces according to its terminal
ddNTP. The successsion of the fluorescences is recorded.
There are two
types of automated sequencing machine in our Faculty. The are
produced by Perkin-Elmer and Beckman-Coulter.